Fig. 3. Neurogenic function of Oct3/4 is not explained by the inhibition of cell autonomic Bmp signaling. MAP2 staining (green) of ZHTc6 cells cultured for 8 days with (A,C,E,G) or without (B,D,F,H) Tc. In some cultures, Bmp2 (10 ng/ml; C,D), BmpßR-Fc (150 ng/ml; E,F), or 10% serum (G,H) were added. Nuclei were stained with Hoechst 33258 (blue). Scale bar, 50 µm.