Fig. 4. Effective SDIA-induced neurogenesis of ES cells accompanied by maintenance of Oct3/4. Expression EB5 ES cells were cultured on O/F-coated dishes (A,C,E) or a monolayer of PA6 cells (B,D,F). Cells were stained with anti-nestin (red) and anti-MAP2 (green) antibodies on each day indicated in the figure. Scale bar, 50 µm. (G) RT-PCR analysis of the expression of Oct3/4 and a mesencephalic dopaminergic neuron marker, Nurr1, in EB5 ES cells cultured as in A-F.