Fig. 5. Requirement of Oct3/4 for SDIA-induced neurogenesis. (A,B) ZHBTc4 and (C,D) ZHTc6 cells were cultured on a PA6 monolayer in serum-free LIF-deficient medium with (B,C) or without (A,D) Tc for 8 days. The cells were stained with an anti-MAP2 antibody (green) and Hoechst 33258 (blue). In ZHBTc4 Oct3/4 expression can be shut off by adding Tc. (E) MAP2-positive colonies were counted. Scale bar, 100 µm.