Fig. 7. Temporal requirement of Oct3/4 for SDIA-induced neurogenesis. ZHBTc4 cells were cultured on a PA6 monolayer for 8 days and stained with anti-nestin and anti-MAP2 antibodies. (A) Tc was added to the medium on and after the indicated days to shut off the Oct3/4 expression until the end of culture. (B) Frequencies of nestin- and MAP2-positive colonies with respect to the total number of colonies are indicated. Vertical bars indicate the s.d. (C) Representative views of cultured ZHBTc4 cells immunostained on indicated days (anti-nestin, red; anti-MAP2, green). Scale bar, 100 µm.