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Fig. 5. HGF and several ECMs regulate C/EBP expression during stem cell differentiation. (A) When cells were cultured on type IV collagen-coated dishes for 8 days, C/EBP{alpha} expression was promoted in a dose-dependent manner by HGF, as well as by the presence of laminin, type IV collagen and type I collagen. By contrast, C/EBPß expression was decreased in these culture conditions. All data were normalized to the expression values from 0 ng/ml (HGF) or non-coated dishes (ECM) and fold differences are shown. Representative data from a stem cell clone are shown; three samples were examined for each concentration of HGF or each ECM. Data are mean±s.d. (B) C/EBP{alpha} was highly expressed in ALB– and ALB+ sorted cells cultured with HGF for 10 days. Its expression was promoted exclusively in the transition of ALB– cells to ALB+ hepatic precursors induced by HGF. All data were normalized to the value of no-GF and fold differences are shown. Representative data from a transfected stem cell clone are shown; three samples were examined for each GF. Data are mean±s.d.