Fig. 6. Ectopic expression of floral homeotic genes in leaves of AtMSI1-CS plants. RNA was isolated from (A) floral buds and open flowers of wild-type plants and leaves of wild-type and AtMSI1-CS, and (B) flowers of wild-type and leaves of fas1, fas2 and fas1 fas2 plants before bolting. After treatment with DNAseI, RNA was subjected to reverse transcription in the presence (+) or absence (-) of reverse transcriptase using oligo(dT) primers. PCR with different cDNA-specific primers was performed on aliquots of the produced cDNA (10 ng total RNA from flowers and 50 ng total RNA from leaves).