Fig. 1. Identification of sra-13. (A) Physical map of the dpy-10 unc-4 interval on chromosome II. The YAC and cosmid clones tested are shown. The three long-PCR fragments derived from the cosmid F49E12 that were tested are shown at the bottom. The F49E12.4, F49E12.5 (sra-13) and F49E12.6 PCR products spanned positions 27,000 to 30,140; 29,500 to 33,085; and 34,600 to 38,300 in the F49E12 cosmid sequence, respectively. `-' indicates no suppression and `+' indicates suppression of the Muv phenotype. (B) Sequence alignment of SRA-13 with SRA-11 (gi2500866), SRA-4 (gi1176649) and SRA-6 (gi1176651). Conserved residues are indicated by the grey shading. The regions underlined in bold are the predicted transmembrane domains. The broken line indicates the extent of deletion in the sra-13 open reading frame in the zh13 allele. (C) Dendogram of the SRA family of GPCRs calculated with the neighbour-joining method using the CLUSTALX software (Thompson et al., 1997).