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Fig. 5. Penetrance of the vulval lin-11::GFP expression at different developmental stages in two transgenic lines: syIs80 (A-C) and syIs53 (D,E). x-axis is the vulval cells, whereas the y-axis is the percentage of animals expressing GFP (sample size for each set is 50). (A) GFP penetrance is higher in P5.pp and P7.pa cells compared with others. syIs53 animals do not reveal vulval expression at this stage. (B,D) By Pn.pxx stage, P5.p and P7.p N,T cells express high levels of GFP. The penetrance is complete in syIs80 animals but lower (60-70%) in syIs53 animals. (C,E) By Pn.pxxx stage, GFP expression is detected in all 2° lineage cells.