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Fig. 8. Expression pattern of ldb-1 during vulval development. Thick arrows indicate vulval opening. (A,C,E,G) Nomarski images, (B,D,F,H) GFP fluorescence photomicrographs. Number of animals examined at each stage are 28 (Pn.px), 25 (Pn.pxx), 34 (early Pn.pxxx), 26 (mid Pn.pxxx), 15 (late Pn.pxxx) and 13 (adult). (A,B) Pn.pxx stage animal expressing faint ldb-1::GFP in vulval progeny. Vulval lineages are drawn. (C,D) An early-L4 stage animal, arrows indicate some of the cells expressing GFP. (E,F) A mid-L4 stage animal. Arrowheads indicate all 1° lineage cells in this focal plane that are expressing GFP. (G,H) A young adult expressing GFP in all the 2° lineage cells. (I) Penetrance of ldb-1::GFP expression at different stages. Anterior is towards the left. Scale bar: 10 µm.