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Fig. 9. Effect of ldb-1 RNAi on vulval morphology and marker gene expression. Vulval cells are marked with thin arrows. Thick arrows indicate the position of the vulval opening. Nomarski images showing morphological defects in the vulva during mid (A,C) and late (E) L4 stages. (B,D,F) GFP fluorescence photomicrographs of the corresponding animals. (A,B) P7.p lineage presumptive vulC fails to express egl-17::GFP (ayIs4). (C,D) Only presumptive vulD, but not presumptive vulC, vulE and vulF, are expressing cdh-3::GFP (syIs50). (E,F) Presumptive vulC of P5.p and P7.p lineage, and presumptive vulB1 of the P5.p lineage fail to express ceh-2::GFP (syIs54). Anterior is towards the left. Scale bar: 10 µm.