Fig. 3. PINCH protein distribution in developing muscle and other tissues. (A) Dorsal view of a stage 16 embryo. PINCH protein can be seen in the developing dorsal vessel (DV; the two rows of immunoreactive cells at the midline) and the pharyngeal musculature (PM). Also note enrichment of PINCH at the muscle-attachment sites (MAS), where the somatic muscles attach to each other and to the epidermal tendon cells. (B) Optical section through a stage 16 embryo. PINCH protein is prominent in the gut, and in the pharyngeal musculature and the visceral muscle (VM) surrounding the gut. (C) Higher magnification of PINCH localization in the pharyngeal, somatic and cardiac muscle lineages. (D) Higher magnification of PINCH in the digestive tract. PINCH is prominent in both the visceral musculature surrounding the gut and the gut epithelial cells (EC).