Fig. 5. GLD-1 depletion disrupts regulation of endogenous GLP-1 expression. Animals were injected with gld-1 dsRNA and then stained for GLP-1 protein by immunofluorescence after incubation for 15 hours at 25°C. Gonads are outlined in A and B. (A) A control gonad dissected from a non-injected animal. GLP-1 staining was restricted to the mitotic proliferation zone of the germline (white bracket). (B) A gonad dissected from a gld-1(RNAi) animal. GLP-1 staining extends beyond the mitotic region (bracket) of the distal arm. This gonad also contained oocytes (arrowheads) with diakinesis nuclei, as judged by DAPI staining (not shown). (C) Quantitation of the expansion of GLP-1 staining from the distal tip, following gld-1(RNAi). The number of nuclei were counted from the distal tip to where GLP-1 staining was lost from peripheral membranes in individual gonads. Numbers represent mean±s.d. from three experiments (n=8-17 gonads for each experiment). (D) A 4-cell embryo from a non-injected control animal. (E) A 4-cell embryo from a gld-1(RNAi) animal. The two posterior blastomeres are indicated (arrowheads) in D and E.