Fig. 2. Expression of CCAP RNA in CCAP neurons, and use of CCAP-GAL4 fusion for targeted ablation of CCAP neurons. (A,B) Expression of CCAP-IR (A) and of CCAP RNA (B), in 3rd instar larva CNS. Neurons located in similar positions are indicated by the same symbols, emphasizing the similarity of the two patterns of expression. (C,D) CCAP-IR (brown) in combination with CCAP RNA expression (blue), illustrating co-localization of these two signals in the 2 pairs of CCAP-immunoreactive neurons in the brain (arrowheads in A and B). (D) Higher magnification of boxed pair of neurons in C; the 2 neurons are very close to each other. Arrowheads point to (clear) nuclei; blue staining is due to RNA expression in the cell bodies, while brown is CCAP-IR, and is especially visible in the neuronal processes (asterisk in C and D). (E) Pattern of CCAP-IR (red and upper right panel) and ß-gal-IR (green and lower right panel) in late 2nd instar CNS of CCAP-GAL4 x UAS-lacZ progeny. All CCAP-immunoreactive neurons were ß-gal immunoreactive, and vice versa. (F) Targeted ablation of CCAP neurons. Pattern of CCAP-IR (red) and ß-gal-IR (green) in CNS of CCAP-GAL4; UAS-rpr + lacZ late 2nd instar. Br, brain; vns, ventral nervous system. Scale bar: (A) 80 µm, (E) 40 µm.