Fig. 5. CCAP and ETH are released at pupation. (A) Pattern of CCAP-IR in the CNS of a pre-pupa. (B-G) Enlargement of boxed area in A, showing (B-D) CCAP-IR in descending axons (arrows) and (E-G) ETH-IR in Inka cell before pre-ecdysis (B,E), at start of pre-ecdysis (C,F) and immediately after (D,G) pupal ecdysis. (H) Quantitation of the intensity of CCAP-IR in descending axon (arrow in B-D) and of ETH-IR. Before, before pre-ecdysis (as in B,E); Pre, at the start of pre-ecdysis (as in C,F); Ecd, immediately after pupal ecdysis (as in D,G). Values are averages±s.e.m.; 8-10 preparations were scored for each time point. Scale bars: 40 µm (D); 10 µm (G).