Fig. 7. aPKC is apically localised and asymmetrically inherited during the perpendicular divisions. (A,B,E) Stage 8 aPKC localisation. (C,F) Stage 8 occludin localisation. (D) No primary antibody negative control. (G,H) Stage 8 aPKC and a tubulin double staining. (I) Transmitted light image of fertilised egg. (J,K) aPKC localisation in fertilised egg. In this case, a pigmented embryo was used to allow identification of animal hemisphere. (L,M) aPKC staining at the four-cell stage. (N) No primary antibody negative control at the four-cell stage. (A-H) No vitelline membrane. In I-M, embryos with vitelline membrane are shown (as they were less damaged), but the staining was the same in those without vitelline membrane. All images are from antibody stained sections, except G,H, which are stained as whole-mount preparations.