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Fig. 6. TUNEL staining of whole-mount wild-type (left) and no soul mutant (right) embryos. C,D,G,H,K,L are higher magnification views of A,B,E,F,I,J, respectively. (A-D) 24 hpf embryos showing comparable TUNEL staining in wild-type and no soul mutant embryos. (E-H) 28 hpf embryos showing increased TUNEL staining in the anterior lateral cranial placodal region. (I-L) 36 hpf embryos showing increased TUNEL staining in the posterior cranial placodal region. MHB, mid-hindbrain boundary; ov, otic vesicle. Scale bar: 100 µm (A,B,E,F,I,J), 50 µm (C,D,G,H,K,L).