Fig. 1. VegT-depleted vegetal cells fail to sort from control animal cap and control vegetal cells. Control vegetal cells injected with RLDX were dissected from stage 9 embryos, dissociated and mixed with unlabelled dissociated animal cap cells (A) or dissociated vegetal cells (B). Aggregates were fixed after 4 hours in culture and viewed by confocal microscopy. Control vegetal cells sort out from animal cells (A) but not from other vegetal cells (B). (C,C',D,D') Dissociated, RLDX-labelled VegT-depleted vegetal cells from stage 9 embryos were mixed with unlabelled control animal cells (C,C') or unlabelled control vegetal cells (D,D'). VegT-depleted vegetal cells fail to sort out in either case and remain randomly distributed.