Fig. 4. Xlim5 does not change germ layer-specific gene expression in vegetal endoderm explants. Vegetal masses were dissected from stage 9 embryos either uninjected (Un Vg) or injected with 1 ng of Xlim5 RNA (Xlim5 Vg) and cultured until stage 22. Explants were assayed by real-time RT-PCR as described above. Note that ectoderm (NCAM, Epidermal keratin and E-cadherin) and mesoderm (Muscle actin) are not induced in Xlim5-injected explants. Endoderm markers [Endodermin (Edd) and Xsox17a] are not significantly affected. Relative expression was determined versus stage 22 whole embryos.