Fig. 7. SMAD1 and ß-catenin are associated in molecular complexes in TgALK3^QD/QD (QD/QD) mice. Molecular associations in postnatal kidney tissue (P10) isolated from different mice were assayed by immunoprecipitation-immunoblot assays using specific antisera. Immunoblots and quantitation are shown in the left and right panels, respectively. (A) The amount of ß-catenin associated with SMAD1 was increased ninefold in Tg mice versus controls. (B) The amount of phospho-SMAD1 associated with ß-catenin was increased ninefold in Tg mice versus controls. (C) The amount of SMAD4 associated with ß-catenin was increased twofold in Tg mice versus controls. Data are mean±s.d. from three independent experiments.