Fig. 1. Generation of microarray data. (A) Schematic representation of the K14MycER expression cassette (top) and immunofluorescence staining (bottom) of wild-type and transgenic epidermis with an antibody to the murine estrogen receptor (HL7), showing expression of transgene in basal layer of epidermis and outer root sheath of hair follicles after 4 days treatment with OHT. No signal is observed in skin of wild-type mice. (B) Model (top) for effects of Myc on the epidermis: red arrows indicate that differentiation into interfollicular epidermis and sebocytes is stimulated. Red cross indicates that stem cell renewal is inhibited. Histology (bottom) of skin 4 days after daily treatment with OHT. Note thickening of interfollicular epidermis (IFE) and enlarged sebaceous glands (SG) in transgenic relative to wild-type control skin. (C) List of the 6 treatment groups subjected to microarray analysis. Skin was harvested after 1 day or 4 days of daily OHT treatment or was untreated (0d). HF, hair follicle. Scale bars: 10 µm.