Fig. 3. (A) RNA expression profile of upregulated genes in skin of untreated (0d) and treated (1d and 4d) transgenic and wild-type mice. (B,C) Immunolabeling of 4 days OHT-treated skin with antibodies to the proteins indicated. Scale bars: 10 µm (B), 5 µm (C). (D) In situ hybridisation of BSSP using radiolabelled RNA antisense probes (BSSP-as), comparing wild-type (wt) and transgenic (tg) skin untreated (0d) or harvested 1 day or 4 days (1d, 4d) after OHT treatment. A ß-actin probe served as positive control and a BSSP sense probe (BSSP-s) as negative control. The arrowheads indicate the expression of BSSP mRNA in sebaceous glands in untreated transgenic mice (tg-0d) and in the interfollicular epidermis of transgenic mice treated with OHT for 4 days (tg-4d). Scale bar: 10 µm