Fig. 7. Myc activation did not disrupt cell-cell adhesion or focal adhesions, but did decrease hemidesmosome formation. (A) Cultured human keratinocytes (Kq-MycER, Kq-106ER, Kq-pBP) were stained with antibodies to the proteins shown. Nuclei are stained for Myc using 9E10 antibody. (B) Flow cytometry of primary keratinocytes isolated from transgenic or wild-type mice 2 days after OHT treatment using antibodies against the ß1 and 6 integrin subunits and CD98. (C) Real-time PCR determination of 6 integrin subunit mRNA levels in transgenic (tg) mice untreated (0d) or treated with OHT for 4 days (4d) (P<0.01).