Fig. 3. Distribution of cls/sox10 response elements in the nac/mitfa promoter. Schematic diagram represents the 836 b.p. nac/mitfa promoter luciferase reporter construct (nac>luc) and derivative constructs with truncations of the nac/mitfa promoter. Co-transfection of the cls/sox10 expression plasmid pCS2sox10 into NIH3T3 cells with these constructs led to higher levels of induction with the full length promoter and incrementally lower levels with incremental 5' truncations of the promoter. Sox10 induction was measured as: (luciferase activity with co-transfected pCS2sox10)/(luciferase activity with co-transfected pCS2sox10L142Q). The values shown are means±s.e.m. from at least four repetitions of each experiment.