Fig. 3. Molecular defects of gp150¹ and gp150². (A) PCR amplification revealed smaller genomic sequences from gp150 mutations than from wild type. Lane 1, l (2)k08316/Df (2R)02311; lane 2, l (2)k00212/Df (2R)02311; lane 3, gp1502/Df (2R)02311; lane 4, gp1501/Df (2R)02311. All four mutant chromosomes were isolated from the same screen (Torok et al., 1993). Both l (2)k08316 and l (2)k00212 were used as positive controls as these two lethal mutations are located outside of the deleted region in Df (2R)02311. The gp150 gene was mapped within the deleted region in Df (2R)02311 (Fetchko et al., 2002). (B) Sequence analysis detected a deletion extending from exon 5 into exon 6 of the gp150 gene. The deleted chromosome was predicted to encode a truncated protein (see text for details).