Fig. 1. TGIF is represented in Drosophila by Achi and Vis. (A) Sequence comparison shows the high TGIF similarity within the homeodomain and extending into the C-domain. The TALE amino acids, RYN, characteristic of the TGIF family of TALE homeodomains, are boxed. The extended helix 3 is shown as predicted by PSIpred. The vertical line indicates the COOH-terminal limit of the homeodomain; ^* indicates identical residues or highly conserved substitutions in all sequences;. indicates less conserved sequences and! indicates conservation only between Achi/Vis and TGIF2. (B) Achi and Vis are highly similar and comparison with the Anopheles TGIF homologue (AgCP3385) indicates conserved regions in addition to the homeodomain and C domain. ^* indicates identity;: indicates highly conserved and. indicates conserved residues. The extents of the homeodomain and C domain are indicated above the sequence. The position of the insertion of exon 6 sequence is indicated by arrowhead. Arrows: number 1 indicates start of deletion in vis^Z3922 and number 2 indicates site of stop codon in achi^Z3922. (C) Exon 6 sequence from class 2 transcripts. Exon 6 sequence is identical in achi and vis.