Fig. 8. Expression of esc in Df(2R)achi¹ mutants. (A) RT-PCR analysis on RNA samples from wild-type and Df(2R)achi¹ mutant testes reveal that esc is amongst the genes showing enhanced transcript abundance in Df(2R)achi¹ mutants, other enhanced transcripts include haywire and Taf24. (B) Anti-Esc immunolabelling on wild-type testes. Esc accumulates in nuclear speckles during primary spermatocyte differentiation. (C) Anti-Esc immunolabelling on Df(2R)achi¹ testis. Esc is clearly expressed and demonstrates the expansion of the early primary spermatocyte population. Nuclear speckles do not form.