Fig. 2. Vis and Achi are widely expressed proteins. Immunofluorescent images of Vis/Achi expression (green) in an embryo (A), a wing imaginal disc (C), an egg chamber (D), and a testis (E). All images are of wild-type tissues except for B which shows a Df(2R)pingpong homozygous mutant embryo. Only background staining is observed, confirming the specificity of the antibody. In C, the inset shows detail of wing disc expression co-stained with propidium iodide (red) to highlight the nuclei. In D, the nurse cell (N), follicle cell (F) and oocyte (O) nuclei are indicated (red arrowhead, oocyte nucleus). In E, the testes are co-stained for Comr (red), which has a very similar expression pattern to Vis/Achi in the testes. Three stages observed in wild-type testes, primary (1°) spermatocytes, elongating spermatids (s) and mitotically dividing germ cells (m) are indicated.