Fig. 5. Relationship between vis, achi and other meiotic arrest genes. (A,B) boule in situ hybridization in pingpong and pingpong/CyO (wild type) testes. boule is not expressed in pingpong testes. (C,D) twine in situ hybridization in pingpong and pingpong; P{achi} (wild type) testes. twine is not expressed in pingpong testes. (E,F) mst87F in situ hybridization in pingpong and

pingpong/CyO (wild type) testes. mst87F is not expressed in pingpong testes. (G,H) Aly protein expression in pingpong and pingpong; P{achi} (wild type) testes. Aly is expressed and is found in the nucleus in pingpong testes. The arrows point to high levels observed apically in the wild type and the more uniform levels observed throughout the testes in the mutant. (I,J) Comr protein expression in pingpong and pingpong; P{achi} (wild type) testes. Comr is expressed and is found in the nucleus in pingpong testes. The arrows point to high levels observed apically in the wild type and the more uniform levels observed throughout the testes in the mutant. (K-P) Vis/Achi protein expression. (K,L) aly¹ and aly¹/CyO (wild type) testes. Vis/Achi is expressed in aly testes. The aly¹ flies were raised at 25°C, where no protein is observed (White-Cooper et al., 2000). The arrows point to high levels observed apically in the wild type and the more uniform levels observed throughout the testes in the mutant. (M,N) sa¹ and sa¹/CyO (wild type) testes. Vis/Achi is expressed in sa testes. The arrows point to high levels observed apically in the wild type and the more uniform levels observed throughout the testes in the mutant. (O,P) can¹² and can¹²/CyO (wild type) testes. Vis/Achi is expressed in can testes. The arrows point to high levels observed apically in the wild type and the more uniform levels observed throughout the testes in the mutant.