Fig. 7. AchiL can rescue pingpong function. (A,B) Immunoblot analysis of Vis/Achi proteins in wild type (WT), pingpong, pingpong; P{vis}, pingpong; P{achi}, and pingpong; tub-AchiL. Germline (O, ovaries; T, testes) and somatic tissues (soma; F, female adult soma; M, male adult soma) were analyzed. In extracts from wild-type testes, two bands are observed, the lower of which is also observed in ovaries. In both male and female somatic tissues a single band is observed that appears to migrate slightly slower than the 60 kDa germline band; the reason for this difference is not known. However, none of these bands are observed in extracts from the pingpong mutant, confirming that they are derived from these genes. These images were cut just above a background band that was present in all lanes. The lower panels in both A and B show each blot stained for total proteins to illustrate that similar amounts of lysate were loaded in each lane (see also Methods). (C,D) pingpong; tub-AchiL testes appear normal by phase contrast microscopy and by immunostaining for Vis/Achi (blue), DNA (red) and CycB (green).