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Fig. 2. (A,B) Single oocyte of Micrura alaskensis continuously bathed in 20 µM U0126. (A) single confocal plane (left) and compressed z-series (right) before GVBD. (B) Same oocyte in mature state 2 hours after treatment with 5-HT plus U0126, showing ER clusters: single optical plane view (left) and compressed z-series (right). (C) Compressed z-series of a mature Cerebratulus sp. oocyte with ER clusters 2 hours after treatment with 5-HT+U0126. Scale bars: 50 µm. GV, germinal vesicle.