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Fig. 2. The hnd-1 gene encodes the C. elegans Hand transcription factor. (A) hnd-1 (formerly C44C10.8) genomic organization. White, untranslated regions; gray and black, coding region; black, basic helix-loop-helix (bHLH) domain. Brackets below mark end points of hnd-1 deletion. (B) Amino acid sequence alignment of bHLH domains of human (h), Xenopus (x), zebrafish (z), Drosophila (Dm) and C. elegans (Ce) HAND proteins. (C) hnd-1 reporters. Top, hnd-1(FL)::GFP inserts GFP after amino acid 163. Middle, hnd-1(N)::GFP fuses GFP to the N terminus of HND-1 and uses the unc-54 3'UTR (light gray). Bottom, hnd-1::GFPlacZ replaces most of the hnd-1 coding region with a GFPlacZ fusion and the unc-54 3'UTR (light gray). GFP and GFPlacZ are not to scale.