Fig. 5. Gonadal primordia in hnd-1 L1 larvae. All images are hnd-1(RNAi). Black arrow, SGP with name of cell; asterisk, PGC. (A-C) pes-1::GFP marks SGPs, GFP overlays DIC image. (A,B) Primordium with two SGPs. Right focal plane shows Z1 (A). Left focal plane shows Z4 (B). (C) Primordium with one SGP, left focal plane. (D) Primordium with no SGPs, two PGCs are present, but are separated. (E-J) lag-2::GFP marks SGPs. (E-G) Two SGPs in normal positions. Z1 is at anterior pole on right (E), Z4 is at posterior pole on left (F). Z1 and Z4 extend cytoplasmic processes to meet mid-ventrally (G, white arrow). (H-J) Primordium with one misplaced SGP. Z1 is displaced dorsally (H), Z4 is located at the posterior pole (I). Z1 and Z4 meet mid-dorsally via a thin cytoplasmic process (J, white arrow). Scale bar: 5 µm.