Fig. 6. hnd-1::GFP expression. (A) Lineage diagram depicting cells that express hnd-1::GFP (green). Dashed lines indicate approximate stage of embryos in panels B-D. (B-H) Confocal images of embryos expressing hnd-1::GFP (green). (B-D) Projections of z-series; embryos were observed over time to identify cells. (B) hnd-1(N)::GFP expression is first detected in four granddaughters of MS, descendants of MS.ap and MS.pp, in four granddaughters of C, descendants of C.ap and C.pp (C.xp), and in two daughters of D. (C) hnd-1(FL)::GFP expression in granddaughters of MS.ap/MS.pp and daughters of C.ap/C.pp. (D) hnd-1(N)::GFP expression fades after the next division of most hnd-1-expressing cells but is retained in daughters of MS.appp and MS.pppp, and in daughters of C.ppp and C.app. (E-H) Embryos fixed and stained with -HLH-1 or -PGL-1 (red). (E) Unlike HLH-1 (red), which is detected in body muscle lineages throughout embryogenesis (Krause et al., 1990), hnd-1(N)::GFP is absent from body muscles by the comma-stage of embryogenesis; at this time, expression is seen in Z1/Z4. (F) hnd-1(N)::GFP is detected in Z1 and Z4 as they meet the PGCs (Z2, Z3), marked by PGL-1 (red). (G) Shortly after, expression is absent from Z1 and Z4. (H) hnd-1 embryos express hnd-1(N)::GFP in Z1/Z4.