Fig. 1. (A) ClustalX alignment of the predicted amino acid sequences of SpAlx1 and LvAlx1. Homeodomains, OAR domains, and charged domains are boxed. Note that in each case the assignment of the start methionine is provisional, as there are three in-frame AUG codons near the 5' end of the open reading frame (ORF) of each mRNA. There are in-frame stop codons upstream of each ORF. (B) Unrooted neighbor-joining tree showing the relationship of Lv/SpAlx1 to other Paired-class proteins (homeodomains only). The other proteins shown are those with Paired-class homeodomains that are most closely related to those of the Cart1/Alx3/Alx4 subfamily (Galliot et al., 1999). A multiple alignment was generated using ClustalX and tree construction was carried out with PAUP^*4.0 (Sinauer Associates) using the Neighbor Joining Method. Numbers indicate the fraction of amino acid substitutions between nodes.