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Fig. 6. Activation of Alx1 expression in transfating cells. L. variegatus embryos were fixed 10 hours after PMC removal and immunostained with {alpha}Alx1 and mAb 6a9. (A) {alpha}Alx1 staining. (B) MAb 6a9 staining. (C) Overlay of A and B. 6a9-positive cells also have nuclear Alx1 (arrows). Some Alx1-positive cells are not stained with 6a9, perhaps because they are at an early stage in the transfating process.