Fig. 6. Activation of Alx1 expression in transfating cells. L. variegatus
embryos were fixed 10 hours after PMC removal and immunostained with
Alx1 and mAb 6a9. (A)
Alx1 staining. (B) MAb 6a9 staining. (C)
Overlay of A and B. 6a9-positive cells also have nuclear Alx1 (arrows). Some
Alx1-positive cells are not stained with 6a9, perhaps because they are at an
early stage in the transfating process.