Fig. 3. Cellular morphology of sling and glial wedge cells filled with biocytin during current-clamp recordings. In cellular recordings of the type shown in Fig. 2, the recording pipette was used to fill cells with biocytin. (A,B) E17 filled cells (red) within the sling (A) and glial wedge (B, arrow). Slices were doubled labeled with GFAP to identify the glial wedge (green labeling in B). (C,D) Cells at P0 within the sling showed a more mature neuronal morphology with multiple dendrites (red). The cell in D is shown double labeled with NeuN (arrow indicates a yellow nucleus). Scale bar: 20 µm.