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Fig. 4. Birth dating of the sling. Coronal sections of brains from animals injected with BrdU on either E14 (A) E15 (B) or E16 (C) and sacrificed at E17. Animals were also injected at E17 (D) or E18 (E) and sacrificed at P0 or injected at P2 and sacrificed at P3 (F). All sections were processed for BrdU immunohistochemistry using a fluorescent (Cy3) secondary antibody. Arrows in B and F show the position of the sling. Scale bar: 250 µm in all panels.