Fig. 2. stbm, fz and fmi are required for apicolateral asymmetric localisation of polarity proteins. Confocal images of pupal wings at about 28 hours. Distal is right and anterior is upwards. (A-F) Loss-of-function clones; mutant tissue indicated by absence of lacZ staining (red, left panels). (G-J) Clones of overexpression indicated by lacZ staining (red, left panels). Note that in all genotypes except fmi, the clones also have significant non-autonomous effects on planar polarity that leads to ectopic localisation of polarity proteins in cells surrounding the clone. (A) Fz-GFP in stbm^Vang-A3. (B) Stbm-YFP in fz²⁵. (C) Fmi in stbm^Vang-A3. (D) Stbm-YFP in fmi^E59. (E) Dsh in stbm⁶. (F) Pk in stbm⁶. (G) Stbm-YFP in Act-GAL4/UAS-Fz overexpression clone. (H) Stbm-YFP in Act-GAL4/UAS-Fmi overexpression clone. (I) Fz-GFP in Act-GAL4/UAS-Stbm overexpresssion clone. (J) Pk in Act-GAL4/UAS-Stbm overexpression clone.