Fig. 3. pk and dsh are required for asymmetric localisation and accumulation of polarity proteins. Confocal images of pupal wings at about 28 hours. Distal is rightwards and anterior is upwards. (A-F) Loss-of-function clones, mutant tissue indicated by absence of lacZ staining (red, left panels). (G-J) Clones of overexpression indicated by lacZ staining (red, left panels, H,I) or Pk staining (red, left panels, G,J). (A) Stbm-YFP in dsh³. (B) Fmi in dsh³. (C) Fz-GFP in pk-sple¹³. (D) Stbm-YFP in pk-sple¹³. (E) Fmi in pk-sple¹³. (F) Fmi in pk-sple¹³ stbm⁶ double mutant clone. Note that Fmi is ectopically localised in cells surrounding clone, owing to the non-autonomous phenotype of stbm clones in the wing (Taylor et al., 1998). (G) Fmi in Ptc-GAL4/UAS-Pk wing at compartment boundary. (H) Stbm-YFP in Act-GAL4/UAS-Pk overexpression clone. (I) Stbm-YFP in Act-GAL4/UAS-Dsh overexpression clone. (J) Fmi in Ptc-GAL4/UAS-Pk; fz¹⁵/fz²³ wing at compartment boundary.