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Fig. 5. In situ hybridization detection of osteoblast marker expression in femurs at E16.5 and P7. (A,D) Bright-field images, von Kossa stain. (B,C,E,F,G-L) Dark-field images. (B,E) Cbfa1 expression at E16.5. (C,F) osteopontin expression at E16.5. (G,H) collagen type I expression at P7. (I,J) osteopontin expression at P7. (K,L) osteocalcin expression at P7. (A,B,C,G,I,K) Sections from a control littermate. (D,E,F,H,J,L) Sections from a Fgfr2cko mouse.