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Fig. 4. FGF signaling in the recombinants. (A) FGF3, FGF4 and FGF8 are expressed in the DLMZ cultivated in isolation, from stage 10.25 to stage 14 (lanes 3-6; DLMZ dissected at stage 10.25 and cultivated up to the stage indicated). (B) FGFRs are differentially expressed in the DLMZ-AC (lane 5) and DMZ-AC (lane 7) recombinants. In particular, FGFR1 and FGFR4a expression is maintained at stage 18 in AC-DMLZ recombinants (lane 5). (C) Isolated animal caps do not express FGF3, FGF4 or FGF8. (D) SU5402 treatment does not suppress FGFR1 and FGFR4a expression in the recombinants.