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Fig. 2. Spen protein is severely reduced in spen14C2 clones. (A-C) Confocal images of a third instar eye imaginal disc containing spen14C2 clones generated by ey-FLP. Clones were marked by the absence of nuclear GFP (A) and stained for Spen (B); the merged image is shown (C). Spen signal is predominantly nuclear (C) and is greatly reduced in spen14C2 clones.