Fig. 2. Spen protein is severely reduced in spen14C2 clones.
(A-C) Confocal images of a third instar eye imaginal disc containing
spen14C2 clones generated by ey-FLP. Clones were
marked by the absence of nuclear GFP (A) and stained for Spen (B); the merged
image is shown (C). Spen signal is predominantly nuclear (C) and is greatly
reduced in spen14C2 clones.