Fig. 8. Dorsocross regulates the patterning of the dorsolateral ectoderm. (A) Fluorescent double-staining with anti-Doc3+2 antibodies (green) and anti-Wg (red). After an initial overlap of expression (see Fig. 4E), at stage 11 there is complementary expression of Doc and wg as the wg stripes become interrupted in the dorsolateral ectoderm. (B,C) Anti-Wg staining (brown; blue anti-ß-galactosidase staining indicates TM3, eve-lacZ-balanced control embryos) of stage 11 embryos. Note persistence of continuous stripes in DocA mutants (B, arrowhead), which contrasts with interrupted stripes in control embryos (C, arrowheads). (D) Expression of Dorsocross (anti-Doc3+2, green) and ladybird (anti-Lbe, red) in the embryonic trunk at stage 11/12. Doc patches are observed between Lb patches similar to the spatial arrangement seen in A. (E,F) Anti-Lbe staining (plus anti-ß-galactosidase staining, blue) of stage 14 embryos. (E) Continuous lb stripes are visible in the ectoderm of DocA mutants. (F) Normal lb expression pattern with separated dorsal patches and ventral stripes in the ectoderm in control embryos. Small groups of cells expressing lb in the lateral region correspond to muscle precursors.