Fig. 2. Dissociation of the descendant cells of the isolated B4.1 blastomeres at the 16-, 32- and 64-cell stages. After dissociation, cells were cultured as partial embryos until hatching stage. Then endoderm formation was detected by expression of alkaline phosphatase (ALP) (A), and muscle formation was monitored by expression of myosin (B). Endoderm formation was suppressed, and every partial embryo developed into muscle.