Fig. 5. Constitutive wg signaling causes follicle cell over-proliferation and abnormal accumulation of Hts protein on the membranes of follicle cells. All egg chambers and ovarioles are labeled for lacZ (red), Hts (green) and nuclei (blue). The micrographs in A-C represent one confocal cross section of an ovariole or an egg chamber, whereas the micrograph in D is one confocal section along the surface of an egg chamber. Marked mutant follicle cell clones are identified by loss of lacZ expression (outlined in A,C,D). (A) An ovariole showing abnormal accumulation of extra mutant Axn somatic cells (outlined) between egg chambers that also express higher levels of Hts than wild-type ones. (B) An ovariole carrying only marked mutant sgg^M1-1 follicle cells. Extra mutant sgg follicle cells accumulate abnormally between egg chambers and break germline cysts into small clusters (arrows). (C) Egg chambers with mutant Axn follicle cell clones that express higher levels of Hts (outlined). (D) A stage-8 egg chamber with a mutant sgg clone in which mutant follicle cells have abnormal morphology and higher levels of Hts expression. Scale bars: 10 µm.