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Fig. 3. Seed and embryo defects. (A) Silique from a wild-type Columbia plant with normal green developing seeds filling the entire length of the silique. (B,C) Siliques from (B) an E1-/-E2+/+ plant and (C) an E1+/+E2-/- plant with developing seeds similar to those of the wild-type silique. (D) Silique from an E1+/-E2-/- plant with the green seeds containing the normal embryos and brown seeds harboring the aborted embryos. Note the significant number of empty spaces between developing seeds resulting from unfertilized ovules. (E) Differential interference contrast (DIC) microscopy of a normal sibling embryo at the torpedo stage. (F) DIC image of an E1+/-E2-/- embryo arrested at the globular stage. (G,H) DIC images showing patterning defects in the suspensor and embryo proper. Arrows point to suspensors.