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Fig. 9. Immunoblotting of wild type, mutant and antisense lines. (A) Total protein extracts were prepared from 7-day-old seedlings and subjected to immunoblotting. Lane 1: wild-type plant; lanes 2-4, antisense lines; lane 5, E1-/-E2+/+ (titan 3) plants; lane 6, E1+/+E2-/- plants; lane 7, E1+/-E2-/- plants. (B) Coomassie Blue-stained gel of the protein extracts used in A to show equal loading.