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Fig. 3. SYN1/DIF1 immunolocalization during meiosis in wild type and swi1-2 mutant. In each panel, the upper picture shows a DAPI-stained nucleus (blue) and the lower one shows the SYN1/DIF1 immunolocalization (red) on the same nucleus. (A-D) Wild-type nuclei. (A) Pre-leptotene nucleus, SYN1/DIF1 is associated with chromatin. (B) Leptotene, SYN1/DIF1 localizes along chromosome axes. (C) Pachytene, SYN1/DIF1 immunolocalization stains synapsed chromosomes. (D) Metaphase I, a weak SYN1/DIF1 signal is detected on condensed bivalents. (E-H) swi1-2 meiotic cells. (E) SYN/DIF1 localizes on chromatin as it does in wild type. (F) SYN1/DIF1 surrounds the 10 condensing univalents. (G) While sister cohesion is released, SYN1/DIF1 remains on the chromatids. (H) A polyad, SYN1/DIF1 localizes in the four nuclei. Scale bars: 10 µm.