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Fig. 4. RAD51 immunolocalization during meiosis in wild type and swi1-2 mutant. RAD51 appears in green and the DAPI-stained DNA in blue. (A) Wild-type leptotene, RAD51 immunolocalization reveals numerous spots on chromosomes. (B) Wild-type pachytene, the number of RAD51 spots has decreased. (C-F) swi1-2 meiotic cells. (C) Early meiotic cells, no RAD51 signal. (D) 10 condensing univalents, no RAD51 signal. (E) 20 free chromatids, no RAD51 signal. (F) A polyad, RAD51 appears in the two nuclei. Scale bars: 10 µm.