(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Expression of differentiation markers in mutant animals. DIC (A,C,E) and corresponding GFP fluorescence (B,D,F) images of ubc-18; lin-35(RNAi) mutant animals. White and black arrowheads indicate anterior and posterior pharyngeal boundaries, respectively. (A,B) Expression of the differentiated muscle cell marker, myo-2::GFP. (C,D) Expression of the adherens junction component and marker for epithelial cell differentiation, ajm-1::GFP. Bracket indicates the region of ajm-1 expression in the non-extended pharynx. (E,F) Neuronal marker (unc-119::GFP) expression in a pharynx that underwent partial extension. White arrows (F) indicate the positions of several GFP+ nuclei that lie within the pharyngeal borders. Scale bar: 10 µm.